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Sysmex Corporation giemsa staining kit diff-quick
Giemsa Staining Kit Diff Quick, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa-diff-quick/giemsa+staining+kit+diff+quick/pm40361486-63-15-19
Average 90 stars, based on 1 article reviews
giemsa staining kit diff-quick - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Transcription Factor Nrf2 Regulates Inflammation by Mediating the Effect of 15-Deoxy-? 12,14 -Prostaglandin J 2
Article Snippet: Differential leukocyte counts were determined in cytospin smears stained with Wright-Giemsa stain (Diff-Quick; Sysmex, Kobe, Japan).

Article Title: Cryobiopsy with endobronchial ultrasonography using a guide sheath for peripheral pulmonary lesions and DNA analysis by next generation sequencing and rapid on-site evaluation.
Article Snippet: Background: The purpose of this study was to evaluate the diagnostic accuracy of Cryo with endobronchial ultrasonography using a guide sheath (EBUS-GS) for peripheral pulmonary lesions (PPLs) to assess the volume of specimen, determine DNA sequencing analysis, and evaluate the utility of rapid on-site evaluation (ROSE).. Methods: Out of 30 patients assessed for eligibility, 23 were enrolled in this prospective study.. The histological diagnostic yield of Cryo was evaluated and the volume was compared to that of trans-bronchial biopsy (TBB).

Article Title: MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells
Article Snippet: After 48 h incubation, cells migrating into the lower chamber, containing 10% FBS as a chemo-attractant, were fixed and stained with Wright-Giemsa stain (Diff-Quick; Sysmex, Kobe, Japan).

Article Title: Transcription Factor Nrf2 Regulates Inflammation by Mediating the Effect of 15-Deoxy-Δ 12,14 -Prostaglandin J 2
Article Snippet: Differential leukocyte counts were determined in cytospin smears stained with Wright-Giemsa stain (Diff-Quick; Sysmex, Kobe, Japan).

Staining:

Article Title: Impacts of Dental Follicle Cells and Periodontal Ligament Cells on the Bone Invasion of Well-Differentiated Oral Squamous Cell Carcinoma.
Article Snippet: When cell cultures reached ~90% confluency, DFCs and PDLCs were seeded in the 6-well plates containing one coverslip each well (22 mm × 22 mm; Matsunami Glass Ind., Ltd., Osaka, Japan) at a density of 3 × 105 cells per well and incubated at 37 ◦C. .. After 2 days, the slides attached with cells were gathered and then stained using a Giemsa staining kit (Diff-Quick; Sysmex Corporation, Kobe, Japan) following the manufacturer’s protocol. .. Images were captured using a bright-field microscope (BX51; Olympus Corporation, Tokyo, Japan) at 40× magnification.

Article Title: Plasmodium falciparum Liver Stage Antigen 3 plays a role in completing the final stages of merozoite invasion of red blood cells.
Article Snippet: .. Staining of parasite-infected human RBCs (iRBCs) smears were performed with a Diff-Quick Staining Kit, a modified Giemsa stain (Sysmex, Kobe, Japan), and the parasites were observed using an Olympus BX41 light microscope (OLYMPUS, Tokyo, Japan). ..

Article Title: Mortierella wolfii keratomycosis in a horse.
Article Snippet: *Racehorse Hospital, Ritto Training Center, The Japan Racing Association (JRA), 1028, Misono, Ritto, Shiga 520-3085, Japan; †Clinical Science and Pathobiology Division, Equine Research Institute (ERI), JRA, 321-4, Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan; ‡Racehorse Hospital, Miho Training Center, JRA, 2500-2, Mikoma, Miho, Inashiki, Ibaraki 300-0493, Japan; §Microbiology Division, Tochigi Branch, Epizootic Research Center, ERI, JRA, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan; –National Institute of Health Sciences, 1-18-1, Kami-yoga, Setagaya, Tokyo 158-8501, Japan; and **Center for Fungal Consultation Japan, 13-1, Yukigayaotsuka-cho, Ota, Tokyo 145-0067, Japan

Diff-Quik:

Article Title: Impacts of Dental Follicle Cells and Periodontal Ligament Cells on the Bone Invasion of Well-Differentiated Oral Squamous Cell Carcinoma.
Article Snippet: When cell cultures reached ~90% confluency, DFCs and PDLCs were seeded in the 6-well plates containing one coverslip each well (22 mm × 22 mm; Matsunami Glass Ind., Ltd., Osaka, Japan) at a density of 3 × 105 cells per well and incubated at 37 ◦C. .. After 2 days, the slides attached with cells were gathered and then stained using a Giemsa staining kit (Diff-Quick; Sysmex Corporation, Kobe, Japan) following the manufacturer’s protocol. .. Images were captured using a bright-field microscope (BX51; Olympus Corporation, Tokyo, Japan) at 40× magnification.

Article Title: Plasmodium falciparum Liver Stage Antigen 3 plays a role in completing the final stages of merozoite invasion of red blood cells.
Article Snippet: .. Staining of parasite-infected human RBCs (iRBCs) smears were performed with a Diff-Quick Staining Kit, a modified Giemsa stain (Sysmex, Kobe, Japan), and the parasites were observed using an Olympus BX41 light microscope (OLYMPUS, Tokyo, Japan). ..

Article Title: Mortierella wolfii keratomycosis in a horse.
Article Snippet: *Racehorse Hospital, Ritto Training Center, The Japan Racing Association (JRA), 1028, Misono, Ritto, Shiga 520-3085, Japan; †Clinical Science and Pathobiology Division, Equine Research Institute (ERI), JRA, 321-4, Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan; ‡Racehorse Hospital, Miho Training Center, JRA, 2500-2, Mikoma, Miho, Inashiki, Ibaraki 300-0493, Japan; §Microbiology Division, Tochigi Branch, Epizootic Research Center, ERI, JRA, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan; –National Institute of Health Sciences, 1-18-1, Kami-yoga, Setagaya, Tokyo 158-8501, Japan; and **Center for Fungal Consultation Japan, 13-1, Yukigayaotsuka-cho, Ota, Tokyo 145-0067, Japan

Modification:

Article Title: Plasmodium falciparum Liver Stage Antigen 3 plays a role in completing the final stages of merozoite invasion of red blood cells.
Article Snippet: .. Staining of parasite-infected human RBCs (iRBCs) smears were performed with a Diff-Quick Staining Kit, a modified Giemsa stain (Sysmex, Kobe, Japan), and the parasites were observed using an Olympus BX41 light microscope (OLYMPUS, Tokyo, Japan). ..

Giemsa Stain:

Article Title: Plasmodium falciparum Liver Stage Antigen 3 plays a role in completing the final stages of merozoite invasion of red blood cells.
Article Snippet: .. Staining of parasite-infected human RBCs (iRBCs) smears were performed with a Diff-Quick Staining Kit, a modified Giemsa stain (Sysmex, Kobe, Japan), and the parasites were observed using an Olympus BX41 light microscope (OLYMPUS, Tokyo, Japan). ..

Light Microscopy:

Article Title: Plasmodium falciparum Liver Stage Antigen 3 plays a role in completing the final stages of merozoite invasion of red blood cells.
Article Snippet: .. Staining of parasite-infected human RBCs (iRBCs) smears were performed with a Diff-Quick Staining Kit, a modified Giemsa stain (Sysmex, Kobe, Japan), and the parasites were observed using an Olympus BX41 light microscope (OLYMPUS, Tokyo, Japan). ..

Sterility:

Article Title: Mortierella wolfii keratomycosis in a horse.
Article Snippet: *Racehorse Hospital, Ritto Training Center, The Japan Racing Association (JRA), 1028, Misono, Ritto, Shiga 520-3085, Japan; †Clinical Science and Pathobiology Division, Equine Research Institute (ERI), JRA, 321-4, Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan; ‡Racehorse Hospital, Miho Training Center, JRA, 2500-2, Mikoma, Miho, Inashiki, Ibaraki 300-0493, Japan; §Microbiology Division, Tochigi Branch, Epizootic Research Center, ERI, JRA, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan; –National Institute of Health Sciences, 1-18-1, Kami-yoga, Setagaya, Tokyo 158-8501, Japan; and **Center for Fungal Consultation Japan, 13-1, Yukigayaotsuka-cho, Ota, Tokyo 145-0067, Japan

Saline:

Article Title: Mortierella wolfii keratomycosis in a horse.
Article Snippet: *Racehorse Hospital, Ritto Training Center, The Japan Racing Association (JRA), 1028, Misono, Ritto, Shiga 520-3085, Japan; †Clinical Science and Pathobiology Division, Equine Research Institute (ERI), JRA, 321-4, Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan; ‡Racehorse Hospital, Miho Training Center, JRA, 2500-2, Mikoma, Miho, Inashiki, Ibaraki 300-0493, Japan; §Microbiology Division, Tochigi Branch, Epizootic Research Center, ERI, JRA, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan; –National Institute of Health Sciences, 1-18-1, Kami-yoga, Setagaya, Tokyo 158-8501, Japan; and **Center for Fungal Consultation Japan, 13-1, Yukigayaotsuka-cho, Ota, Tokyo 145-0067, Japan

Incubation:

Article Title: Mortierella wolfii keratomycosis in a horse.
Article Snippet: *Racehorse Hospital, Ritto Training Center, The Japan Racing Association (JRA), 1028, Misono, Ritto, Shiga 520-3085, Japan; †Clinical Science and Pathobiology Division, Equine Research Institute (ERI), JRA, 321-4, Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan; ‡Racehorse Hospital, Miho Training Center, JRA, 2500-2, Mikoma, Miho, Inashiki, Ibaraki 300-0493, Japan; §Microbiology Division, Tochigi Branch, Epizootic Research Center, ERI, JRA, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan; –National Institute of Health Sciences, 1-18-1, Kami-yoga, Setagaya, Tokyo 158-8501, Japan; and **Center for Fungal Consultation Japan, 13-1, Yukigayaotsuka-cho, Ota, Tokyo 145-0067, Japan



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Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) <t>Giemsa</t> <t>staining</t> was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
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Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Journal: International Journal of Oncology

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

doi: 10.3892/ijo.2022.5368

Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

Techniques: Activation Assay, In Vitro, Staining, Immunofluorescence, Expressing

Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Journal: International Journal of Oncology

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

doi: 10.3892/ijo.2022.5368

Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

Techniques: Migration, In Vitro, Staining